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acriia4 abe8e  (Addgene inc)


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    Addgene inc acriia4 abe8e
    Acriia4 Abe8e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acriia4+abe8e/AcrIIA4+(Plasmid+%23101043)/pmc11871365__41467_2025_57154_MOESM1_ESM-184-100-105
    Average 92 stars, based on 7 article reviews
    acriia4 abe8e - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9
    Article Snippet: Gene editors plasmids. plasmid name resource PX549 Addgene #62988 Left-BH-REC12 Derived from PX459, insert BH-REC1-REC2 at N-terminus of REC Left-BH-REC1 Derived from PX459, insert BH-REC1 at N-terminus of REC Right-REC1 Derived from PX459, insert REC1 at c-terminus of REC Right-REC12 Derived from PX459, insert REC1-REC2 at c-terminus of REC Left-Rec12 (=GS-Cas9) Derived from PX459, insert REC1-REC2 at N-terminus of REC SpCas9-P2A-GFP Derived from Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70), fragements SnaBI/ApaI from PX459 were inserted. .. GS-Cas9-P2A-GFP Derived from Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70), fragements SnaBI/ApaI from P182 were inserted. pCMV-TadA-TadA* Addgene #125661 ABE8e Addgene #138489 ABE8e/Left-BH-REC12 or GS-ABE8e-2BH ABE8e, insert BH-REC1-REC2 at N-terminus of REC ABE8e/Right-REC12 ABE8e, insert REC1-REC2 at C-terminus of REC ABE8e/Left-Rec12 (=GS-ABE8e) ABE8e, insert REC1-REC2 at N-terminus of REC ABE8e/Left-0.5BH-Rec12, or GS-ABE8e-1.5BH ABE8e, , insert 0.5BH-REC1-REC2 at N-terminus of REC ABE9 Addgene #194208 ABE9/Left-Rec12 (=GS-ABE9) Derived from ABE9, insert REC1-REC2 at N-terminus of REC dead SaCas9 Addgene #138162 nSaCas9, BA31_pU.CAG.SaCas9-D10A.rBGpA Addgene #199251 Td-CGBE Addgene #196599, bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS Td-CGBE/Left-Rec12 eTd-CBE Addgene #196601, bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI- bpNLS eTd-CBE/Left-Rec12 AcrIIA4 Addgene #86842, pJH376, mammalian expression of AcrIIA4 ABE8e(TadA-8e V106W) or ABE8e(V106W) Addgene #138495 ABE8e(TadA-8e V106W)/Left-Rec12 or GSABE8e(V106W) REC from P102 insert into P275, BglII/ApaI nCas9-GFP Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70) ABE8e-GFP p RZ70 as vector was inserted ABE8e fragment, SnaBi/ApaI GS-ABE8e-GFP p RZ70 as vector was inserted P102 fragment, SnaBi/ApaI SpCas9-2BH PX459 was inserted BH of St1Cas9 at N-terminus of REC ABE8e-2BH ABE8e was inserted BH of St1Cas9 at N-terminus of REC ABE8e-Flag Flag tag DYDDDDK was fused to C-end of Cas9 scaffold GS-ABE8e-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9 scaffold GS-ABE8e-1.5BH-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9-1.5BH scaffold GS-ABE8e-2BH-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9-2BH scaffold BE4max Addgene #112093 GS-BE4max BE4max was inserted St1Cas9 REC domain BE4maxΔUGI 2xUGI was deleted in BE4max GS-BE4maxΔUGI 2xUGI was deleted in GS-BE4max UGI Addgene # 101091 GS-nmeABE8e St1Cas9 REC domain was replaced by Nme1Cas9 REC domain in GS-Cas9 SaABE8e Addgene #138500 GSa-ABE8e St1Cas9 REC domain was inserted between BH domain and REC domain in SaCas9 scafflod CGBE1 Addgene #140252 GS-CGBE1 St1Cas9 REC domain was inserted in CGBE1 ..

    Expressing:

    Article Title: Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9
    Article Snippet: Gene editors plasmids. plasmid name resource PX549 Addgene #62988 Left-BH-REC12 Derived from PX459, insert BH-REC1-REC2 at N-terminus of REC Left-BH-REC1 Derived from PX459, insert BH-REC1 at N-terminus of REC Right-REC1 Derived from PX459, insert REC1 at c-terminus of REC Right-REC12 Derived from PX459, insert REC1-REC2 at c-terminus of REC Left-Rec12 (=GS-Cas9) Derived from PX459, insert REC1-REC2 at N-terminus of REC SpCas9-P2A-GFP Derived from Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70), fragements SnaBI/ApaI from PX459 were inserted. .. GS-Cas9-P2A-GFP Derived from Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70), fragements SnaBI/ApaI from P182 were inserted. pCMV-TadA-TadA* Addgene #125661 ABE8e Addgene #138489 ABE8e/Left-BH-REC12 or GS-ABE8e-2BH ABE8e, insert BH-REC1-REC2 at N-terminus of REC ABE8e/Right-REC12 ABE8e, insert REC1-REC2 at C-terminus of REC ABE8e/Left-Rec12 (=GS-ABE8e) ABE8e, insert REC1-REC2 at N-terminus of REC ABE8e/Left-0.5BH-Rec12, or GS-ABE8e-1.5BH ABE8e, , insert 0.5BH-REC1-REC2 at N-terminus of REC ABE9 Addgene #194208 ABE9/Left-Rec12 (=GS-ABE9) Derived from ABE9, insert REC1-REC2 at N-terminus of REC dead SaCas9 Addgene #138162 nSaCas9, BA31_pU.CAG.SaCas9-D10A.rBGpA Addgene #199251 Td-CGBE Addgene #196599, bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS Td-CGBE/Left-Rec12 eTd-CBE Addgene #196601, bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI- bpNLS eTd-CBE/Left-Rec12 AcrIIA4 Addgene #86842, pJH376, mammalian expression of AcrIIA4 ABE8e(TadA-8e V106W) or ABE8e(V106W) Addgene #138495 ABE8e(TadA-8e V106W)/Left-Rec12 or GSABE8e(V106W) REC from P102 insert into P275, BglII/ApaI nCas9-GFP Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70) ABE8e-GFP p RZ70 as vector was inserted ABE8e fragment, SnaBi/ApaI GS-ABE8e-GFP p RZ70 as vector was inserted P102 fragment, SnaBi/ApaI SpCas9-2BH PX459 was inserted BH of St1Cas9 at N-terminus of REC ABE8e-2BH ABE8e was inserted BH of St1Cas9 at N-terminus of REC ABE8e-Flag Flag tag DYDDDDK was fused to C-end of Cas9 scaffold GS-ABE8e-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9 scaffold GS-ABE8e-1.5BH-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9-1.5BH scaffold GS-ABE8e-2BH-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9-2BH scaffold BE4max Addgene #112093 GS-BE4max BE4max was inserted St1Cas9 REC domain BE4maxΔUGI 2xUGI was deleted in BE4max GS-BE4maxΔUGI 2xUGI was deleted in GS-BE4max UGI Addgene # 101091 GS-nmeABE8e St1Cas9 REC domain was replaced by Nme1Cas9 REC domain in GS-Cas9 SaABE8e Addgene #138500 GSa-ABE8e St1Cas9 REC domain was inserted between BH domain and REC domain in SaCas9 scafflod CGBE1 Addgene #140252 GS-CGBE1 St1Cas9 REC domain was inserted in CGBE1 ..

    FLAG-tag:

    Article Title: Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9
    Article Snippet: Gene editors plasmids. plasmid name resource PX549 Addgene #62988 Left-BH-REC12 Derived from PX459, insert BH-REC1-REC2 at N-terminus of REC Left-BH-REC1 Derived from PX459, insert BH-REC1 at N-terminus of REC Right-REC1 Derived from PX459, insert REC1 at c-terminus of REC Right-REC12 Derived from PX459, insert REC1-REC2 at c-terminus of REC Left-Rec12 (=GS-Cas9) Derived from PX459, insert REC1-REC2 at N-terminus of REC SpCas9-P2A-GFP Derived from Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70), fragements SnaBI/ApaI from PX459 were inserted. .. GS-Cas9-P2A-GFP Derived from Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70), fragements SnaBI/ApaI from P182 were inserted. pCMV-TadA-TadA* Addgene #125661 ABE8e Addgene #138489 ABE8e/Left-BH-REC12 or GS-ABE8e-2BH ABE8e, insert BH-REC1-REC2 at N-terminus of REC ABE8e/Right-REC12 ABE8e, insert REC1-REC2 at C-terminus of REC ABE8e/Left-Rec12 (=GS-ABE8e) ABE8e, insert REC1-REC2 at N-terminus of REC ABE8e/Left-0.5BH-Rec12, or GS-ABE8e-1.5BH ABE8e, , insert 0.5BH-REC1-REC2 at N-terminus of REC ABE9 Addgene #194208 ABE9/Left-Rec12 (=GS-ABE9) Derived from ABE9, insert REC1-REC2 at N-terminus of REC dead SaCas9 Addgene #138162 nSaCas9, BA31_pU.CAG.SaCas9-D10A.rBGpA Addgene #199251 Td-CGBE Addgene #196599, bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS Td-CGBE/Left-Rec12 eTd-CBE Addgene #196601, bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI- bpNLS eTd-CBE/Left-Rec12 AcrIIA4 Addgene #86842, pJH376, mammalian expression of AcrIIA4 ABE8e(TadA-8e V106W) or ABE8e(V106W) Addgene #138495 ABE8e(TadA-8e V106W)/Left-Rec12 or GSABE8e(V106W) REC from P102 insert into P275, BglII/ApaI nCas9-GFP Addgene #123616, NLS-nCas9-NLS-P2A-EGFP (pRZ70) ABE8e-GFP p RZ70 as vector was inserted ABE8e fragment, SnaBi/ApaI GS-ABE8e-GFP p RZ70 as vector was inserted P102 fragment, SnaBi/ApaI SpCas9-2BH PX459 was inserted BH of St1Cas9 at N-terminus of REC ABE8e-2BH ABE8e was inserted BH of St1Cas9 at N-terminus of REC ABE8e-Flag Flag tag DYDDDDK was fused to C-end of Cas9 scaffold GS-ABE8e-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9 scaffold GS-ABE8e-1.5BH-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9-1.5BH scaffold GS-ABE8e-2BH-Flag Flag tag DYDDDDK was fused to C-end of GS-Cas9-2BH scaffold BE4max Addgene #112093 GS-BE4max BE4max was inserted St1Cas9 REC domain BE4maxΔUGI 2xUGI was deleted in BE4max GS-BE4maxΔUGI 2xUGI was deleted in GS-BE4max UGI Addgene # 101091 GS-nmeABE8e St1Cas9 REC domain was replaced by Nme1Cas9 REC domain in GS-Cas9 SaABE8e Addgene #138500 GSa-ABE8e St1Cas9 REC domain was inserted between BH domain and REC domain in SaCas9 scafflod CGBE1 Addgene #140252 GS-CGBE1 St1Cas9 REC domain was inserted in CGBE1 ..



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    acriia4 abe8e  (Addgene inc)
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    Addgene inc acriia4 abe8e
    Acriia4 Abe8e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acriia4+abe8e/AcrIIA4+(Plasmid+%23101043)/pmc11871365__41467_2025_57154_MOESM1_ESM-184-100-105
    Average 92 stars, based on 1 article reviews
    acriia4 abe8e - by Bioz Stars, 2026-09
    92/100 stars
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